Maaß T, Bayley CP, Mörgelin M, Lettmann S, Bonaldo P, Paulsson M, Baldock C, Wagener R

J. Biol. Chem. 2016 Mar;291(10):5247-58

PMID: 26742845


Collagen VI, a collagen with uncharacteristically large N- and C-terminal non-collagenous regions, forms a distinct microfibrillar network in most connective tissues. It was long considered to consist of three genetically distinct α chains (α1, α2, and α3). Intracellularly, heterotrimeric molecules associate to form dimers and tetramers, which are then secreted and assembled to microfibrils. The identification of three novel long collagen VI α chains, α4, α5, and α6, led to the question if and how these may substitute for the long α3 chain in collagen VI assembly. Here, we studied structural features of the novel long chains and analyzed the assembly of these into tetramers and microfibrils. N- and C-terminal globular regions of collagen VI were recombinantly expressed and studied by small angle x-ray scattering (SAXS). Ab initio models of the N-terminal globular regions of the α4, α5, and α6 chains showed a C-shaped structure similar to that found for the α3 chain. Single particle EM nanostructure of the N-terminal globular region of the α4 chain confirmed the C-shaped structure revealed by SAXS. Immuno-EM of collagen VI extracted from tissue revealed that like the α3 chain the novel long chains assemble to homotetramers that are incorporated into mixed microfibrils. Moreover, SAXS models of the C-terminal globular regions of the α1, α2, α4, and α6 chains were generated. Interestingly, the α1, α2, and α4 C-terminal globular regions dimerize. These self-interactions may play a role in tetramer formation.

  • Upcoming Events

    1. Cologne Seminars on Ageing Series 2018 with Elaine Fuchs

      März 6 @ 4:00 pm - 5:30 pm
    2. SFB 829 Connector Meeting

      April 6 @ 3:00 pm - 5:30 pm
    3. SFB 829 Connector Meeting

      August 31 @ 3:00 pm - 5:30 pm
    4. International Symposium „Molecular Mechanisms regulating Skin Homeostasis“

      November 12 - November 14
    5. SFB 829 Connector Meeting

      Dezember 7 @ 3:00 pm - 5:30 pm
  • News

© 2017 SFB 829