LL37:DNA complexes provide antimicrobial activity against intracellular bacteria in human macrophages

Stephan A, Batinica M, Steiger J, Hartmann P, Zaucke F, Bloch W, Fabri M

Immunology 2016 May;

PMID: 27177697

Abstract

As part of the innate host response neutrophils release neutrophil extracellular traps (NETs), protein:DNA complexes that contain a number of antimicrobial peptides (AMPs), such as cathelicidin. Human cathelicidin in its active form, LL37, has potent antimicrobial activity against bacteria. However, whether LL37 derived from NETs contributes to antimicrobial activity against intracellular pathogens remains unclear. Here, we report that NETs induced by mycobacteria contain cathelicidin. Human macrophages internalized NET-bound cathelicidin, which is transported to lysosomal compartments. Furthermore, using a model of in vitro-generated LL37:DNA complexes we found that LL37 derived from such complexes attacks mycobacteria in macrophage phagolysosomes resulting in antimicrobial activity. Taken together, our results suggest a mechanism, by which LL37 in complex with DNA contributes to host defense against intracellular bacteria in human macrophages. This article is protected by copyright. All rights reserved.

  • Upcoming Events

    1. 3rd Connector Meeting

      April 12 @ 2:30 pm - 5:00 pm
    2. 4th Connector Meeting

      September 6 @ 3:00 pm - 6:30 pm
    3. 5th Connector Meeting

      November 8 @ 3:00 pm - 6:00 pm
  • News

© 2017 SFB 829

Durch die weitere Nutzung der Seite stimmst du der Verwendung von Cookies zu. Weitere Informationen

Die Cookie-Einstellungen auf dieser Website sind auf "Cookies zulassen" eingestellt, um das beste Surferlebnis zu ermöglichen. Wenn du diese Website ohne Änderung der Cookie-Einstellungen verwendest oder auf "Akzeptieren" klickst, erklärst du sich damit einverstanden.

Schließen